Phale, P.S.

Dr. Prashant S. Phale
Professor

Phone: +(91-22) 2576 7836
E-mail: pphale [at] iitb.ac.in
Location: Room No. 405, BSBE Building

Research Interest

  • Elucidation of metabolic pathways for degradation of aromatic compounds, Purification and characterization of oxygenases, Preferential utilization of aromatics compounds by Pseudomonas, Metabolic engineering of aromatic degradation pathways 

Academic Background

  • B.Sc. Ahemadnagar college, University of Poona, Poona (Microbiology)
  • M.Sc. Maharaja Sayajirao University, Baroda (Biotechnology)
  • Ph.D. Indian Institute of Science, Bangalore (Microbiology)

Teaching (current)

Analytical Biochemistry, Biochemistry Laboratory, Microbiology Laboratory

Professional Experience

  • Associate Professor: School of Biosciences & Bioengineering, IIT Bombay
  • Assistant Professor : School of Biosciences & Bioengineering, IIT Bombay
  • Post Doctoral Research Fellow: Harvard Medical School, Boston, USA
  • Post Doctoral Fellow: Biozentrum, University of Basel, Switzerland  

Memberships

  • Life member of:

    • National Academy of Sciences, India
    • Society of Biological Chemists, India
    • Association of Microbiologist of India
    • Biotechnology Research Society of India

Key Publications

    • Trivedi VDJangir PK, Sharma R, & Phale PS (2016) Insights into functional and evolutionary analysis of carbaryl metabolic pathway from Pseudomonas sp. strain C5pp. Sci. Rep6, 38430; doi: 10.1038/srep38430.
    • Pandey S, Modak A, Phale PS* & Bhaumik P* (2016) High resolution structures of periplasmic glucose binding protein of Pseudomonas putida CSV86 reveal structural basis of its substrate specificity. J Biol Chem 291:7844–7857;  *Corresponding authors
    • Paliwal V, Raju SC, Modak A, Phale PS & Purohit HJ (2014) Pseudomonas putida CSV86: A candidate genome for genetic bioaugmentation. PLoS One 9(1):e84000. doi: 10.1371/journal.pone.0084000.
    • Modak A, Bhaumik P & Phale PS (2014) Periplasmic glucose binding protein from Pseudomonas putida CSV86: Identification of the glucose binding pocket by homology-model-guided site-specific mutagenesis. FFEBS J 281:365-375.
    • Singh R, Trivedi VD & Phale PS (2013) Metabolic regulation and chromosomal localization of carbaryl degradation pathway in Pseudomonas sp. strains C4, C5 and C6. Arch Microbiol 195:521-535.
    • ShrivastavaR, Basu B, Godbole A, MathewMK, ApteSK & Phale PS (2011) Repression of glucose inducible outer membrane protein, OprB, during utilization of aromatic compounds and organic acids in Pseudomonas putida CSV86. Microbiology 157:1531-1540.
    • Sah S & Phale PS (2011) 1-Naphthol 2-hydroxylase from Pseudomonas sp. strain C6: purification, characterization and chemical modification studies. Biodegradation 22:517-526.
    • BVamsee-Krishna C & Phale PS (2010) Bypassing isophthalate inhibition by modulating glutamate dehydrogenase: purification and kinetic characterization of NADP-GDHs from isophthalate degrading Pseudomonas aeruginosa strain PP4 and Acinetobacter lwoffii strain ISP4. J Bacteriol 192:801–806.
    • Deveryshetty J, & Phale PS (2009) Biodegradation of phenanthrene by Pseudomonas sp., strain PPD: purification and characterization of 1-hydroxy 2-naphthoic acid dioxygenase. Microbiology 155:3083-3091.
    • Basu A, ShrivastavaR, BasuB, ApteSK & PhalePS (2007) Modulation of glucose transport causes preferential utilization of aromatic compounds in Pseudomonas putida CSV86. J Bacteriol 189:7556-7562.
    • Swetha VP, Basu A & Phale PS (2007) Purification and characterization of 1-naphthol-2-hydroxylase from carbaryl degrading Pseudomonas strain C4. J Bacteriol 189:2660-2666.
    • Vamsee-Krishna C, Mohan Y & Phale PS (2006) Biodegradation of phthalate isomers by Pseudomonas aeruginosa PP4, Pseudomonas sp. PPD and Acinetobacter lwoffii ISP4. Appl Microbiol Biotechnol 72:1263-1269.
    • Basu A, Apte SK & Phale PS (2006) Preferential utilization of aromatic compounds over glucose by Pseudomonas putida CSV86. Appl Environ Microbiol 72:2226-2230.
    • Swetha VP & Phale PS (2005) The metabolism of carbaryl via 1,2-dihydroxy naphthalene by soil isolates, Pseudomonas sp. strain C4, C5 and C6. Appl Environ Microbiol 71:5951-5956.
    • Prabhu Y & Phale PS (2003) Biodegradation of phenanthrene by Pseudomonas sp. strain PP2: novel metabolic pathway, role of biosurfactant and cell surface hydrophobicity in hydrocarbon assimilation. Appl Microbiol Biotechnol 61:342-351.

The metabolic pathways contributed to ‘The University of Minnesota Biocatalysis/Biodegradation Database’