BB407: Analytical Biochemistry

Separation techniques in biochemistry: Centrifugation, TLC and Paper chromatography, gel permeation, ion exchange, hydrophobic, reverse-phase and affinity chromatography, HPLC and FPLC, Electrophoretic techniques, Criteria of protein purity, equilibrium dialysis, ultrafiltration and various membrane techniques, API-electrospray and MALDI-TOF, Mass spectrometery, Radiotracer techniques for enzyme assays, receptor-ligand interactions; radioimmunoassay, ELISA.

Text/References :

  • D. Holme & H. Peck; Analytical Biochemistry. Longman, 1983.
  • T.G. Cooper; The Tools of Biochemistry. Wiley Intersciences, 1977.
  • R. Scopes; Protein Purification – Principles & Practices. Springer Verlag, 1982.
  • Selected readings from Methods in Enzymology, Academic Press.
  • R.C. Price, Proteins. Lafbax Academic Press 1996.