Tayalia, P

Dr. Prakriti Tayalia

Associate Professor

Phone: +(91-22) 2576 7797
Fax: +(91-22) 2572 7760
E-mail: prakriti [at] iitb.ac.in
Location: Room No. 602, BSBE Building
Lab web page

Post-Doctoral position available for candidates with a background and experience in immunology.
The candidate will get a hands-on experience to work in the interdisciplinary field of materials and immunology and will be able to focus on basic as well as translational research. Specific responsibilities will include planning and performing experiments, writing grants, giving guidance to project/Ph.D students.

Research Interest

Our lab has progressively been developing various kinds of material based systems for replicating and understanding in vivo physiological phenomena. With that aim, we are working with three-dimensional (3D) cell culture platforms both for delivery of bioactive molecules as well as recruitment or culture of cells, which can broadly be used for the following applications.
(1)  Material-based gene delivery: We have an in house expertise of making lentiviral particles for different kinds of genetic modification of cells. We have tried a variety of plasmids and have used different material platforms for delivery of these particles to cells in vitro as well as in vivo. We can transduce different cells using various direct or material based approaches to manipulate cells ex vivo or allow in situ (or in vivo) genetic modification of cells respectively. We are using this platform for immunotherapy and tissue regeneration applications.
(2)  Porous cryogel scaffolds for 3D cell culture: We have also developed a macroporous hydrogel platform for immunotherapy and spheroid formation.

  1. Immunotherapy: These porous matrices can be loaded with programming factors such as growth factors (or chemokines) and gene delivery vectors, which can be temporally controlled and used to recruit and manipulate host cells upon in vivo implantation. This approach when used in a more cell specific manner with immune cellshas applications in immunotherapy.
  2. Tissue regeneration: Our cryogel matrices are also being developed as an off the shelf degradable macroporous matrix system for full thickness skin regeneration. We plan to develop this system as a substitute for the currently used decellularized cadaveric skin, autografts or other expensive allogeneic cell based or acellular matrices imported in the Indian market.
  3. Spheroid formation: By modulating the composition of these porous matrices, we can grow clusters or spheroids of various kinds of cells. These 3D cell culture platforms can be used (1) to understand underlying cellular mechanisms in normal and pathological conditions and (2) for drug screening applications by exploring cell-cell and cell-ECM interactions, influence of immune cells on tumor cells and vice versa.

Better understanding of cellular mechanisms in various applications will enable these material based systems developed in our group to be taken forward for some real clinical applications.

Education

  • 2009 : Ph.D. in Applied Physics and Bioengineering, Harvard University, Cambridge, USA
  • 2002 : M.S. in Materials Science & Engineering, University of Delaware, Newark, USA
  • 1999 : B.Tech. in Metallurgical Engineering and Materials Science, Indian Institute of Technology, Bombay, India

Academic Experience

  • Associate Professor (2019 – present): Department of Biosciences and Bioengineering, IIT Bombay, Mumbai, India
  • Assistant Professor (2011 – 2019): Department of Biosciences and Bioengineering, IIT Bombay, Mumbai, India
  • Postdoctoral Fellow (2009 – 2011): School of Engineering and Applied Sciences, Harvard University, Cambridge, USA

Honors and Fellowships

  • Department of Biotechnology – Innovative Young Biotechnologist Award (DBT–IYBA) 2013
  • Young Faculty Award, IIT Bombay.
  • Graduate Student Fellowship, Harvard University, USA
  • Awarded Medal for M.S. research work at the 47th International SAMPE Symposium and Exhibition (ISS&E).
  • Recipient of the prestigious National Talent Search Scholarship awarded by NCERT, New Delhi.
  • Awarded Scholarship and Certificate from Central Board of Secondary Education (CBSE) for being among the top 0.1% in Mathematics and Science in the SSC examination (1993).


Publications

  • Singh A, Tayalia P*. Three-dimensional cryogel matrix for spheroid formation and anti-cancer drug screening. J Biomed Mater Res. 2020, 108(2), 365-376. DOI: 10.1002/jbm.a.36822.
  • Dandia H, Makkad K, Tayalia P*. Glycated collagen – a 3D matrix system to study pathological cell behavior. Biomater. Sci. 2019, 7(8), 3480-3488.
  • Peter M, Singh A, Mohankumar K, Jeenger R, Joge P, Gatne M, Tayalia P*. Gelatin based matrices as tunable platform to study in vitro and in vivo 3D cell invasion. ACS Appl. Bio Mater. 2019, 2(2), 916–929.
  • Das S, Srinivasan S, Srivastava A, Kumar S, Das G, Das S, Dwivedi A, Karulkar A, Makkad K, Bilala R, Gupta A, Sawant A, Nayak C, Tayalia P, Purwar R. Differential Influence of IL-9 and IL-17 on Actin Cytoskeleton Regulates the Migration Potential of Human Keratinocytes. J. Immunol. 2019, 202(7), 1949-1961.
  • Kirti S, Patel K, Das S, Shrimali P, Samanta S, Kumar R, Chatterjee D, Ghosh D, Kumar A, Tayalia P, Maji SK. Amyloid Fibrils with Positive Charge Enhance Retroviral Transduction in Mammalian Cells. ACS Biomater. Sci. Eng. 2019, 5(1), 126–138.
  • Shrimali P, Peter M, Singh A, Dalal N, Dakave S, Chiplunkar SV, Tayalia P*. Efficient in situ gene delivery via PEG diacrylate matrices. Biomater. Sci. 2018, 6, 3241-3250.
  • Peter M, Tayalia P*. An alternative technique for patterning cells on poly(ethylene glycol) diacrylate hydrogels. RSC Adv. 2016, 6, 40878-40885.
  • Madhuri Vinchurkar M, Ashwin M, Joshi A, Singh A, Tayalia P, Rao VR. MEMS aptasensor for label-free detection of cancer cells. 3rd International Conference on Emerging Electronics (ICEE) 2016. DOI: 10.1109/ICEmElec.2016.8074610.
  • Ali OA*, Tayalia P*, Shvartsman D, Mooney DJ. Inflammatory cytokines presented from polymer matrices differentially generate and activate Dendritic Cells in situ. Adv. Func. Mater. 2013, 23(6), 4621–4628.
  • Tayalia P, Mendonca CR, Baldacchini T, Mooney DJ, Mazur E. 3D Cell-Migration Studies using Two-Photon Engineered Polymer Scaffolds. Adv.Mater. 2008, 20, 4494-8.
  • Tayalia P, Mazur E, Mooney DJ. Controlled architectural and chemotactic studies of 3D cell migration. Biomaterials 2011, 32, 2634-41.
  • Tayalia P, Mooney DJ. Controlled Growth Factor Delivery for Tissue Engineering. Adv. Mater. 2009, 21, 3269-85.
  • Malhotra D, Fletcher AL, Astarita J, Lukacs-Kornek V, Tayalia P, Gonzalez SF, et al. Transcriptional profiling of stroma from inflamed and resting lymph nodes defines immunological hallmarks. Nat. Immunol. 2012, 13, 499-U107.
  • Lukacs-Kornek V, Malhotra D, Fletcher AL, Acton SE, Elpek KG, Tayalia P, et al. Regulated release of nitric oxide by nonhematopoietic stroma controls expansion of the activated T cell pool in lymph nodes. Nat. Immunol. 2012, 12, 1096-U105.
  • Correa DS, Tayalia P, Cosendey G, dos Santos DS, Aroca RF, Mazur E, et al. Two-Photon Polymerization for Fabricating Structures Containing the Biopolymer Chitosan. J. Nanosci. Nanotech. 2009, 9, 5845-9.
  • Mendonca CR, Correa DS, Marlow F, Voss T, Tayalia P, Mazur E. Three-dimensional fabrication of optically active microstructures containing an electroluminescent polymer. App. Phys. Lett. 2009, 95, 11.
  • Mendonca CR, Correa DS, Baldacchini T, Tayalia P, Mazur E. Two-photon absorption spectrum of the photoinitiator Lucirin TPO-L. App. Phys. Mater. Sci. Process. 2008, 90, 633-6.
  • Mendonca CR, Correa DS, Voss T, Tayalia P, Mazur E. Fabrication of microstructures containing the conjugated polymer MEH-PPV.  2008 Conference on Lasers and Electro-Optics & Quantum Electronics and Laser Science Conference, 2008, 2441-2.
  • Mendonca CR, Baldacchini T, Tayalia P, Mazur E. Reversible birefringence in microstructures fabricated by two-photon absorption polymerization. J. App. Phys. 2007, 102, 1.
  • Tayalia P, Heider D, Gillespie JW. Characterization and theoretical modeling of magnetostrictive strain sensors. Sensors & Actuators A-Physical. 2004, 111 (2-3), 267-274.