Punekar, NS

Dr. N.S. Punekar


Phone: +(91-22) 2576 7775
Fax: +(91-22) 2572 7760
E-mail: nsp [at] iitb.ac.in
Location: Room No. 404, BSBE Department
Lab web page

Research Interest

Microbial Biochemistry and Molecular Enzymology (Major fields) Microbial Metabolic Regulation, Understanding Metabolism through biochemical & recombinant DNA techniques, Fungal Molecular Genetics and its Applications to Metabolic Engineering

Academic Background

  • B.Sc.1976, Karnatak University, Dharwad
  • M.Sc. 1978, Karnatak University, Dharwad
  • Ph.D. 1984, Indian Institute of Science, Bangalore
Involvement with International Olympiad
  • National Scientific Committee Member – 33rd International Chemistry Olympiad, Mumbai 2001
  • National Scientific Committee Member – 19th International Biology Olympiad, Mumbai 2008


Molecular Enzymology, Industrial microbial metabolism, Research Philosophy

Professional Experience

  • Post Doctoral Fellow :1983-87 : Institute for Enzyme Research, University of Wisconsin, Madison, USA.
  • Senior Research Scientist :1987-88 Research Centre, Hoechst India Limited, Bombay 400 080.
  • Professor : 2001-to date Biotechnology Group, BSBE IIT Bombay.
  • Career Development Fellow :1996-98 : Plant Pathology Dept., Rockefeller Foundation University of Wisconsin, Madison, USA (summers only)
  • Editorial Board Member – IJBB, IJBT, IJEB  (2005 onwards)

Publications (Five most recent)

  • (2019, In Press) Characterization of 4-guanidinobutyrase from Aspergillus niger, Tejaswani Saragadam, Sunil Kumar and Narayan S. Punekar Microbiology
  • (2018) Structural basis for the catalytic mechanism and a-ketoglutarate cooperativity of glutamate dehydrogenase Prem Prakash, Narayan S. Punekar, and Prasenjit Bhaumik J. Biol. Chem. 293:6241–6258
  • (2018) Novel route for agmatine catabolism in Aspergillus niger, Saragadam T., Punekar N.S. 4-Guanidinobutyrase Assay. In: Alcázar R., Tiburcio A. (eds) Polyamines. Methods in Molecular Biology, vol 1694. pp.163-172, Humana Press, New York, NY
  • (2016) Contribution of arginase to manganese metabolism of Aspergillus niger, Sarita Keni, Narayan S. Punekar, Biometals, 29:95–106 DOI 10.1007/s10534-015-9900-6 [Link]
  • (2015) Expression of lactate dehydrogenase in Aspergillus niger for L-Lactic acid production, Khyati Dave, Narayan S. Punekar, PLoS ONE 10(12): e0145459. doi:10.1371/journal.pone.0145459 [Link]


  • ENZYMES: Catalysis, Kinetics and Mechanisms ISBN 978-981-13-0784-3 ISBN 978-981-13-0785-0, Springer Nature 2018

List of Patents

  • Branched chain alkyl esters of 2-[4-(2-piperidino-ethoxy)-benzoyl]-benzoic acid, processes for their preparation and their use as spasmolytic agents. N.S. Punekar and five others from Hoechst India Limited. Indian Patent, 255/BOM/88, September 07, 1988; and European Patent, 88 117 521.0, October 21, 1988
  • Affinity resins for use in protein purification on the basis of anthraquinone dye-ligands. N.S. Punekar and six others from CIBA Specialty Chemicals Limited. European Patent, Patent Priority date, 08.04.2005 (EP)Member, Core Committee of Department of Biotechnology, N.Delhi, for framing National Syllabus for Masters in Biotechnology
  • Novel recombinant protein expression system involving a strong, constitutive promoter of citrate synthase from Aspergillus. N.S. Punekar and Kashyap Dave from IIT-Bombay, Powai, Mumbai. Indian Patent, Patent Application No. 2542/MUM/2009 date of filing 03.11.2009
  • Process for the Production of Lactic Acid from Aspergillus, N.S. Punekar and Khyati Dave from IIT-Bombay, Powai, Mumbai. Indian Patent, Patent Application No. 2077/MUM/2014, date of filing 26.06.2014