computational and experimental approaches
Precision oncology is to define molecular profiles of cancer and treatment based on targetable alterations or a specific factor that drives the disease at molecular levels. Since it is tricky to categorize which mutation drives the tumor growth and which are passengers, we perform computational, biochemical, histological, in vitro, and in vivo analyses to identify those driver genes.
Although genomics and proteomics studies have transformed cancer research, developing mutation-based therapy is no easy task. Targeting these oncogenes-based vulnerabilities with drugs is the aim of precision oncology. Most of the genetic alterations in cancer happen in the tumor-suppressor genes, wherein mutations disable the tumor suppressor function or expression. The tumor suppressors are not produced in the cancer cells, making them difficult to target. Therefore, we target the dependent pathways and devise treatment strategies.
Our computational analysis allows us to predict novel driver genes and their potential roles in cancer using genomics and proteomics studies. However, it can be challenging to determine which gene or mutation drives tumor growth and which are simply passengers.
For further understanding, we use wet-lab tools to define the role of a gene in cancer and explore its molecular mechanisms. These fundamental studies pave the way for more precise treatments. Our near future goal is to create clinically-relevant models using patient tumors of Indian origin so that our findings can have a direct impact on our population.
Development of Liquid Biopsy Assay
Our lab focuses on advancing liquid biopsy technologies to enhance cancer diagnostics and precision oncology. By analyzing circulating tumor DNA (ctDNA) from blood using next-generation sequencing (NGS), we aim to capture intra- and inter-tumoral heterogeneity with high accuracy.
Our innovative approach in NGS library preparation and data analysis seek to improve sensitivity and reliability, enabling early detection and personalized treatment strategies for cancer patients.