ABOUT THE FACILITY
High Resolution Mass Spectrometry based Proteomics Research and Training Facility at Indian Institute of Technology Bombay (MASSFIITB) aims to provide advanced, state-of-the-art, mass spectrometry instrumentation and support to expand omics research, education and training in India. The facility can analyze challenging low-abundant, highly complex samples to identify and quantify compounds in a fast and accurate manner along with a thorough elucidation of metabolite structures using the Orbitrap Fusion Tribrid Mass Spectrometer.
The facility is open to researchers in academia, national R&D Labs, as well as corporate customers. A dedicated team of scientists will be available to help researchers in achieving their scientific goals of proteomics and metabolomics research. During the first phase, services offered include protein identification and protein quantification (e.g. iTRAQ/ TMT/ Label-free) for proteomics analysis. During the second phase, services will be expanded to include targeted proteomics, PTM analysis, metabolomics, and other specialized applications. The facility also aims to facilitate proteomics training by conducting dedicated courses and workshops.
GENERAL INSTRUCTION TO THE USERS
The experiment data is provided is only for research/development purposes. These cannot be used as certificates in legal disputes. The sample details and other details as well as cost will be addressed during experimental discussion. PAYMENT HAS TO BE MADE IN ADVANCE ONLY.
We advise users to be cautious in their sample preparation for mass spectrometry analysis as it is highly sensitive to various contaminants such as PEG (Poly ethylene glycol), keratin and various salts. In order to reduce PEG and keratin in the sample it is recommended to use powder free nitrile gloves. Please supply the sample is a low-salt buffer (less than 10mM).
- Solvents used should be pure as possible and HPLC grade is recommended.
- Avoid volatile solvents! Several of these solvents are common in protein chemistry: glycerol, polyethylene glycol, b-mercaptoethanol, Triton-X-100, DMSO, DMF, etc.
- Original, clean Eppendorf tubes should be used as the plastic can leach and cause contamination in the results.
- Sequencing grade trypsin is recommended.
- It is recommended to use the Coomassie/silver staining Standard protocol.
Levels of buffers and detergents which exceed the following limits will probably cause noticeable degradation of the spectrum.
| Phosphate Buffer | >50mM | SDS | 0.01% |
| Ammonium Bicarbonate | >30mM | Alkali metal salts | >1M |
| Tris buffer | >100mM | Glycerol | >1% |
| Guanidine | >1M | Sodium Azide | >1mM |
| Detergent (eg. Tritox-X) | 0.1% |
SAMPLE CHARGES
"For Sample Submission, Please click here"
CONTACT
Prof. Sanjeeva Srivastava
Email ID: sanjeeva@iitb.ac.in, massfiitb@gmail.com
Phone number: (91-22)2576-4714