The first step in this process of protein identification is the generation of peptides by proteolytic digestion of proteins, either in solution or within the polyacrylamide gel, using specific enzymes such as trypsin. The generated peptides are then subjected to LC-MS and the proteins are identified by comparing the experimentally-determined MS peak mass values with predicted mass values of peptides generated by theoretical digestion of the protein in a database such as MASCOT.
Currently samples can be provided as follows:
1) In buffer solution post-extraction of protein ( Currently we are not accepting samples in this form )
2) Cut-outs from 1 D or 2D, SDS-PAGE separated proteins