Instructions to Users

DBT-funded High Resolution Mass Spectrometry based Proteomics Research and Training Facility, IIT Bombay

Registration:

All users are required to register by following the below mentioned registration process to avail the services at the DBT-funded High Resolution Mass Spectrometry based Proteomics Research and Training Facility, IIT Bombay:

Registration Process:

Internal Users:

Users within IIT Bombay are required to fill in the form available at the facility website. The form should be completely filled and all details should be provided. Users are requested to be present at the time of analysis on the allotted appointment date/time.

External Users:

External users are also required to register and fill in the form at the facility website and provide all essential details about the sample. Any query related to registration or analysis should be emailed to massfiitb@gmail.com.  After registration, you will get an appointment for the same.

Academic Institutions: You are required to register and fill in the form at the facility website and provide all essential details about the sample and also send a letter from the Head/Guide on your College/Institute Original Letter Head for registration stating that the analysis is for research purpose to qualify for academic concession. The letter should be addressed to: Prof-In-Charge, Prof. Sanjeeva Srivastava, DBT-funded High Resolution Mass Spectrometry based Proteomics Research and Training Facility, Biosciences and Bioengineering Department, IIT Bombay, Powai, Mumbai-400076.

Industry & Non-Government Agencies:

You are required to register and fill in the form at the facility website  and provide all essential details about the sample and also send in a letter from your organization for registration. The letter should be addressed to: Prof-In-Charge, Prof. Sanjeeva Srivastava, DBT-funded High Resolution Mass Spectrometry based Proteomics Research and Training Facility, Biosciences and Bioengineering Department, IIT Bombay, Powai, Mumbai-400076. You are requested to mention in your request letter that “We agree to acknowledge the DBT-funded High Resolution Mass Spectrometry based Proteomics Research and Training Facility of IIT Bombay when the data from the lab is used in our papers/reports/thesis”. The information on such acknowledgements with appropriate reference should be communicated to the facility via email at massfiitb@gmail.com. Kindly send the complete publication reference (Journal name/volume number/names of the authors/date of issue of the publication etc.).

Payment:

The final amount will only be finalized after discussion based on the experimental set-up. A rough estimate of the charges applicable are given on the facility website. Payment should be made in advance by a Demand Draft (DD) drawn in favour of “The Registrar, IIT Bombay, P and C Account”. The same should be sent to Prof. Sanjeeva Srivastava, Convener HRMS Facility, Lab #304, Department of Biosciences & Bioengineering, IIT Bombay, Powai, Mumbai – 400076, along with the Request Letter.

Appointment: The users will be informed about their date and time-slot by email. If the day and time-slot is not suitable for you, an email request should be sent immediately for an alternate slot and this will be changed depending on availability.

Sample Submission: Samples to be analyzed should be brought along on the date of your appointment for your sample analysis.

Results: After the sample analysis is complete, the results will be sent by email.

 

GENERAL INSTRUCTIONS TO THE USERS:

The experimental data provided is only for research/development purposes. These cannot be used as certificates in legal disputes. Samples will not be analysed till payment is received. The sample details and other details as well as cost will be addressed during experimental discussion.

We advise users to be cautious in their sample preparation for mass spectrometry analysis as it is highly sensitive to various contaminants such as PEG (poly ethylene glycol), keratin and various salts. In order to reduce PEG and keratin in the sample it is recommended to use powder free nitrile gloves. Please supply the sample in a low-salt buffer (less than 10 mM).

  • Solvents used should be pure as possible and HPLC grade is recommended.
  • Avoid volatile solvents! Several of these solvents are common in protein chemistry: glycerol, polyethylene glycol, b-mercaptoethanol, Triton-X-100, DMSO, DMF, etc.
  • Original, clean Eppendorf tubes should be used as the plastic can leach and cause contamination in the results.
  • Sequencing grade trypsin is recommended
  • It is recommended to use the Coomassie/silver staining Standard protocol.

Levels of buffers and detergents which exceed the following limits will probably cause noticeable degradation of the spectrum

Phosphate Buffer > 50 mM
Ammonium Bicarbonate > 30 mM
Tris Buffer > 100 mM
Guanidine > 1M
Detergent (e.g., Triton-X) 0.1%
SDS 0.01%
Alkali  metal salts > 1M
Glycerol > 1%
Sodium azide > 1mM