About Proteomics

What is Proteomics?
 Proteomics describes the study and characterization of complete set of proteins present in a cell, organ or organism at a given time.  The first genome sequence for a free-living organism was completed in 1995, and subsequently many large-scale genome sequencing projects for a number of organisms including human have been completed. Although the completion of human genome sequencing project was remarkable, it was insufficient to unravel the mysteries of essential biological processes. A more meaningful understanding of gene expression can be achieved through characterization of the products of expression, the proteins, which are essential determinants of biological function. Gene expression is regulated by post-transcriptional and post-translational modifications due to which the number of proteins expressed in a cell are many times larger than their genomic counterpart. The term proteome describes the protein complement expressed by a genome of a given cell at a given time including the set of all protein isoforms and modifications. Analogous to genomics, proteomics describes the study and characterization of complete set of proteins present in a cell, organ or organism at a given time. Several techniques used in proteomics typically aim to elucidate the expression, localization, interaction, structure, biochemical activity, and cellular roles of proteins. Systems biology and proteomics strive to create detailed predictive models for molecular pathways based upon the quantitative behaviour of proteins. While understanding these dynamic networks provides clues into the consequence of aberrant interactions and why they lead to diseases like cancer, collecting biochemical data about protein behaviour at scale has been daunting. Dr. Sanjeeva Srivastava’s Proteomics Research Laboratory at IIT Bombay is using high throughput proteomic techniques such as two-dimensional difference in gel electrophoresis, mass spectrometry and protein microarray etc. for biomarker discovery in cancer & tropical diseases of India such as malaria, to study protein-protein interactions and drug target discovery. Information obtained from the research program is also used for in silico studies and computing models to enhance their understanding in a systems approach.
The Proteomics Laboratory at IIT Bombay
Dr. Sanjeeva Srivastava Group is the Leader of the Proteomics Laboratory at the Indian Institute of Technology Bombay India (IITB). He obtained his Ph.D. from the University of Alberta and further did post-doc from the Harvard Medical School in the area of proteomics, stress physiology and has specialized expertise in the applications of data-enabled sciences in global health, developing countries and resource-limited settings. Current research in his group centers on biomarker and drug target discovery and deciphering the protein interaction networks in complex human diseases (e.g., gliomas) as well as infectious diseases (e.g., malaria) with the use of high throughput proteomics, nanoproteomics, protein microarrays, DIGE and mass spectrometry. Additionally, multi-dimensional Omics data are employed for in silico studies and models. The group has developed E-learning resources such as Virtual Proteomics Laboratory and Open Source Courseware Animations Repository as a community resource and is collaborating actively both across India and internationally to advance this knowledge frontier for the benefit of global health. Dr. Srivastava has taught proteomics courses at the Cold Spring Harbor Laboratory and is the recipient of several awards including the National Young Scientist Award (Canada), Young Scientist Awards (Department of Atomic Energy and Board of Research in Nuclear Sciences India and the Department of Science and Technology India) and the Apple Research Technology Support Award (UK). His name is included in Who’s Who in Medicine and Healthcare in Nov 2011 edition of Marquis Who’s Who, USA.
The Human Proteome Organisation (HUPO)
The Human Proteome Organization (HUPO) is an international scientific organization representing and promoting proteomics through international cooperation and collaborations by fostering the development of new technologies, techniques and training.
The Human Proteome Project
The mission of the Human Proteome Project is to characterize all 20,300 genes of the known genome, generate the map of the protein based molecular architecture of the human body, and become a resource to help elucidate biological and molecular function and advance the diagnosis and treatment of diseases.
Human Pediatric Proteome Project (PEDIOME)
The mission of the Human Pediatric Proteome Project is to characterize the normal pediatric proteome from birth until adulthood, and to coordinate longitudinal disease studies by creating a worldwide paediatric biorepository network and inventory.