PROTEOMICS: Quantitative and Trans-proteomic Analysis



Proteomics is study of protein complements of a given cell at a given time, including the set of all protein isoforms and modifications. Emerging proteomics technologies capable of rapid and ac- curate screening of thousands of proteins are found to be very effective for the multidisciplinary research and these high-throughput technological approaches have propelled their implication in almost all areas of modern life-science research. Various high-throughput integrated proteomics technologies capable of fast and accurate screening of thousands of biomolecules are found to be very effective for studying disease pathobiology and identification of next-generation biomark- ers and potential drug/ vaccine targets, and therefore considered as valuable tools for multidisci- plinary research. Proteomic technologies are rapidly evolving and new technological platforms are coming forward regularly with versatile novel applications; therefore, there is need to keep-up the pace with latest developments in field. In this proteomics course, distinguished faculty scientists involved in advanced proteomics research will deliver lectures and provide training to the students and young researchers. The overall course will be comprised of 10 days enduring lectures and prac- tical courses with a focus on cutting-edge quantitative and targeted proteomic technologies and trans-proteomic pipeline platform.

Modules, Dates and participants Module: Trans-proteomic pipeline (Dr. Robert Moritz)

Proteomics overview: Why Trans-proteomic pipeline? PeptideProphet for statistical valida- tion, InterProphet and PTMProphet, An overview of XPRESS, ASAPRatio and Libra for TPP, Kojak – Protein Cross-linking, SRM and SWATH targeted proteomics, Whole Proteome Re- sources – PeptideAtlas, SWATH analysis of Modifications, Advanced analysis of proteomics data, Advanced SWATH Resources

Module: Quantitative & Targeted proteomics (Drs. Srivastava, Srikanth, Kulkarni) Basics of mass spectrometry and Label-free proteomic techniques, MALDI-TOF/TOF for the PMF and MS/MS analysis, Quantitative proteomics using iTRAQ, iTRAQ protocol & ap- plications, PTM studies using Mass Spectrometry, Case studies: studying glcosylation and phosphorylation using MS, Targeted proteomics applications for glycomics, Application of quantitative proteomics, Metabolomics using LC-MS & GC-MS, Multi-Omics and Systems medicine

Duration: Ten days: 10th to 19th December 2015

No. of participants for the course will be limited to fifty

You should attend If….. This course is designed for last yrs of Bachelor’s/ M.Tech / M.Sc / PhD students of Life sci- ence, Biology, Medicine (with special interest), who are likely to be benefited by learning the fundamental aspects of proteomics and the latest technology. Faculty members and Research Associates are also welcome. This is an excellent opportunity for the participants to learn de- tails of fast growing field in life science.
Fees The participation fees for taking the course are as follows: Participants from abroad : US $250
Full course (10 to 19 December 2015):
Rs. 2000-fees for students (academic institutions)
Rs. 10000-fees for industry participants
Module-I: Trans-Proteomic Pipeline (10 to 14 December 2015)
Rs. 1000-fees for students (academic institutions)
Rs. 5000-fees for industry participants
Module-I: Fee-waiver for TPP workshop participants

Module-II: Quantitative Proteomics using Mass Spectrometry (15 to 19 December 2015)
Rs. 1000-fees for students (academic institutions)
Rs. 5000-fees for industry participants

Additionally, participants have to pay for their hostel accommodation fees in IIT (subject to the availability of hostel).

The Faculty

Dr. Sanjeeva Srivastava, Course Coordinator

Dr. Srivastava is Associate Professor and Group Lead- er of Proteomics Laboratory at the Indian Institute of Technology Bombay India. Dr. Srivastava is recipient of several awards including the National Young Scien- tist Award (Canada), Young Scientist Awards (India) and the Apple Research Technology Support Award (UK). Recently he was awarded the excellence in Teaching Award – 2014 at IIT Bombay. His group has developed E-learning resources such as Virtual Laboratory as a communi- ty resource and is collaborating actively both across India and internation- ally to advance this knowledge frontier for the benefit of global health.

Course Coordinators

Dr. Sanjeeva Srivastava
Principle Coordinator
Associate Professor
Indian Institute of Technology
Telephone: +91 22 2576 7779

Dr. Robert Moritz, Invited International Scientist

Dr. Moritz, is the Professor and Director of Proteom- ics Research at ISB. He has designed and implemented a number of technologies such as TPP, PeptideAtlas, SRMAtlas, currently used in many proteomics labo- ratories across the globe. He is continuing that work at ISB by expanding the ISB proteomics centre into a national facility with online tools for data analysis. Re- cently he was appointed as Vice-president of Human Proteome Organization (HUPO).

Dr. Robert Moritz
Institute for Systems Biology, USA
Vice Precident President, Human Proeome Organization (HUPO)
Dr. Mahesh Kulkarni, Invited National Scientist

Dr. Mahesh Kulkarni is scientist at CSIR-National Chemical Laboratory Pune. He obtained his Ph.D. from University of Agricultural Sciences Bangalore. He was post-doctoral fellow at CCMB, Hyderabad and Genome Institute of Singapore. His area of research is chemical proteomics, mass spectrometry, diabetes and aging.

Dr. Mahesh Kulkarni
National Chemical Laboratory, Pune
Dr. Srikanth Rapole, Invited National Scientist

Dr. Srikanth is working as a scientist at NCCS, Pune. His main research interest is to quantitatively identify the protein signatures involved in human diseases, in- cluding cancer, using mass spectrometry-based pro- teomic approaches. In addition, his group is also work- ing to identify and quantify key metabolites and lipids associated with human diseases using mass spectrom- etry and bioinformatics. He has received best paper award from CSIR in physical sciences. Recently, he received the DBT- Rapid grant for young investigators award.

Dr. Srikanth Rapole
National Center For Cell Sciences, Pune


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