Prof. Sanjeeva Srivastava is actively involved in several teaching activities at IIT Bombay and teaches the following courses:
1. BB 101: Biology
Quantitative views of modern biology. Importance of illustrations and building quantitative/qualitative models. Role of estimates. Cell size and shape. Temporal scales. Relative time in Biology. Key model systems – a glimpse. Management and transformation of energy in cells. Mathematical view – binding, gene expression and osmotic pressure as examples. Metabolism. Cell communication. Genetics. Eukaryotic genomes. Genetic basis of development. Evolution and diversity. Systems biology and illustrative examples of applications of Engineering in Biology.
2. BB 607 : Proteomics: Principles and Techniques
An introduction to proteomics: Basics of protein structure and function, An overview of systems biology, Evolution from protein chemistry to proteomics; Abundance-based proteomics: Sample preparation and prefractionation steps, Gel-based proteomics – two-dimensional gel electrophoresis (2-DE), two-dimensional fluorescence difference in-gel electrophoresis (DIGE), Staining techniques. Quantitative proteomics – stable isotope labeling by amino acids in cell culture (SILAC), isotope-coded affinity tag (ICAT), isobaric tagging for relative and absolute quantitation (iTRAQ); Central role of mass spectrometry: ionization sources, mass analyzers, different types of mass spectrometers; Functional proteomics: Recombinational cloning, Interactomics – techniques to study protein-protein interactions, yeast two-hybrid, immunoprecipitation, protein microarrays, Nucleic Acid Programmable Protein Array (NAPPA), Label-free nanotechnologies in proteomics, Surface Plasmon Resonance (SPR); Modificomics: understanding post-translational modifications; Structural proteomics; Bioinformatics in proteomics; Challenges and future prospects of proteomics research.
Highlights of the course:
Guest speakers: To advance the knowledge of students and to update them with latest technologies, few speakers were invited from industry.